This was a similar pattern of upregulated TNF secretion as that observed in M1 macrophages derived from patients with DADA2 compared with age matched healthy controls (Figure 3D), confirming that our CRISPR/Cas9 THP-1 cell line model closely recapitulated the immunophenotype observed in primary immune cells from patients with DADA2 (1, 2). This evidence concerns the gene TNF and deficiency of adenosine deaminase 2.