SARS-CoV-2 spike-specific CD4+ cell responses, determined as expression of CD69+ and CD134+, were detected in all tested COVID-19 patients (n=15) and vaccinees 6 weeks (n=20), 3 months (n=15) and 6 months after the first vaccine dose (n=17) (Figure 2B). This evidence concerns the gene TNFRSF4 and COVID-19.