To determine whether M-MDSC-like cells are capable of suppressing the T cell response, splenic CD8+ T cells (Supplemental Figure 2A) were activated and co-cultured with CD11b+Ly-6Chi cells sorted from the spleen (Supplemental Figure 2B) at 4 days after JEV infection or JE brain tissue (Supplemental Figure 2C). This evidence concerns the gene ITGAM and Japanese encephalitis.