In the subgroup analysis, there were no associations between plasma OPG levels and functional outcomes at 3 months after index stroke according to sex, age, hypertension, diabetes mellitus status, body mass index, hypercholesterolemia status, coronary artery disease status, congestive heart failure status, atrial fibrillation status, smoking history, alcohol intake, previous stroke history, NIHSS score, or thrombolysis method (mechanical thrombectomy only vs. tPA and mechanical thrombectomy). This evidence concerns the gene TNFRSF11B and Hypercholesterolemia.