To establish whether TBs-C promotes autophagy and induces G1 phase arrest in lung cancer cells by inhibiting the PI3K/AKT/mTOR pathway, the cells were treated with TBs-C (60 μg/mL) after pretreatment with the AKT activator SC79 (10 μg/mL). The gene discussed is AKT1; the disease is lung cancer.