Finally, we analyzed the cell–cell interaction between the microglial cell population (csf1r+/cx3cr1+) and cluster 5 cells identified as neurons (snap25+/syt1+), and it was found that cluster 2 and cluster 3 microglia, under an inflammatory activated state, and neurons also have the same cell–cell interaction relationship between CD74–APP and CD74–COPA that was observed in the hypothalamus of mice injected with ACP cyst fluid (Fig. 5G). Here, CSF1R is linked to cyst.