The control staining with the secondary anti-mouse IgE-HRP antibody showed weaker staining for the corresponding bands (lane 2, 3, 5, 6, 7, 8, 10, 11, Fig. 4D), suggesting that MAGE antigen in addition to blood is also present on some proteins in peritoneal fluids of GC patients. This evidence concerns the gene IGHE and gastric cancer.