FTO and esophageal squamous cell carcinoma: In the next step, FTO was also significantly upregulated in ESCC tissues compared to normal esophageal epithelium tissue based on GSE20347 dataset that microarray analysis of the gene expression profiles of 17 paired ESCC specimens and normal esophageal epithelium tissues (Fig. 1C).Consistently, the immunohistochemical (IHC) staining of the tissue arrays of 28 pairs of ESCC specimens and adjacent normal esophageal epithelial tissues also showed that the expression level of FTO was significantly higher than that of normal tissues (Fig. 1D).