We thus explored the functional impact of Tspan7 expression on the metastatic progression of OS tumors both in vitro and in vivo, revealing that it contributes to tumor cell migratory activity through both the induction of EMT and the interaction with β1 integrin that ultimately results in the activation of FAK-Src-Ras-ERK1/2 pathway. Here, TSPAN7 is linked to neoplasm.