The C15_C0 cluster mainly expressed the macrophage cells marker gene (CD68, CD74) and C15_C1 mainly expressed the astrocyte cells marker genes (CLU, PAX2) (Fig. S7D), illustrating that the immune microenvironment in RB tumors mainly consisted of tumor-associated macrophages (TAMs) and astrocyte-like cells (Fig. 4A), consistent with previous findings [11]. This evidence concerns the gene CLU and neoplasm.