To further explore the underlying mechanism of DCLK1‐mediated cancer stemness and 5‐fluorouracil resistance, we identified CCAR1 as a protein interacting with DCLK1, and elucidated that the C‐terminal domain (Pro301 to Met729) of DCLK1 was indispensable for interacting with CCAR1. This evidence concerns the gene DCLK1 and cancer.