Moreover, in subcutaneous xenograft tumour models treated with 5‐fluorouracil, the volumes of subcutaneous xenograft tumour in DCLK1‐overexpressing group were significantly greater compared to the control (Figure 2E‐a–c), and the TUNEL staining of subcutaneous xenograft tumour was decreased, while the PCNA staining was increased (Figure 2E‐d). Here, DCLK1 is linked to neoplasm.