RAPGEF2 and hepatocellular carcinoma: To further discover the potential influence of RAPGEF2 on HCC progression in vivo, we established human MHCC97 HCC cell stably expressing control (Ctrl) or RAPGEF2 shRNA where the RAPGEF2 protein was successfully knocked down (Figure 3(a)) and implanted MHCC97 cells with or without RAPGEF2 knockdown into BALB/C nu/nu mice.