Dysregulation of these miRNAs can affect inflammation, insulin signaling, response to tissue injury, and lipid metabolism.27 In the current study, trans-chalcone had a hepatoprotective role against NAFLD progression by inhibiting changes in the hepatic expression levels of miR-122, 21, 34a, and 451. The gene discussed is INS; the disease is metabolic dysfunction-associated steatotic liver disease.