In this work, we developed indocyanine green (ICG) labelled recombinant fluorescent annexin V32 probes (ICG–EGFP33–Annexin V and ICG–mPlum34–Annexin V, Scheme 1) that enable both in vitro and in vivo fluorescence imaging of breast-tumour cell apoptosis induced by an ADC, trastuzumab emtansine35 (T-DM1, Kadcyla). This evidence concerns the gene ANXA5 and breast neoplasm.