Western blot analysis demonstrated that the protein levels of LASS2 and TIMP2 were apparently elevated, accompanied by reductions in SPHK1, MMP2, and MMP9 protein levels, in glioma xenografts derived from U-87 MG-pLV-LASS2 cells compared with those derived from U-87 MG-pLV cells (Fig. 5F). This evidence concerns the gene SPHK1 and central nervous system cancer.