Our main findings include: 1) LASS2 is lowly expressed in high-grade gliomas and GSCs; 2) LASS2 overexpression reduced the sphere formation and promoted the differentiation of glioma stem cells; 3) LASS2 overexpression significantly reduced colony formation, migration, and invasion of glioma cells, and 4) Overexpression of LASS2 inhibited U-87 MG cell-derived glioma xenograft growth in nude mice. Here, CERS2 is linked to glioma.