Overexpression of LASS2 reduced the protein expression of SPHK1, MMP9, MMP2, Bcl-2, Vimentin, and N-cadherin while increasing that of TIMP2, Bax, activated caspase3, TNF-α, p53, and E-cadherin both in vitro and in vivo, suggesting that LASS2 suppresses the development and progression of glioma mainly by inhibiting tumor cell migration, invasion, and proliferation 28-30. This evidence concerns the gene TIMP2 and glioma.