Immunohistochemical staining also demonstrated that MMP9 and SPHK1 levels were apparently reduced, but LASS2 and TIMP2 levels were increased in glioma xenografts derived from U-87 MG-pLV-LASS2 cells compared with those derived from U-87 MG-pLV cells (Fig. 5E). The gene discussed is TIMP2; the disease is central nervous system cancer.