TNF and central nervous system cancer: Western blot analysis demonstrated a reduction in the protein level of Bcl-2 and an increase in the protein levels of Bax, cleaved Caspase-3, TNF-α, and p53 in glioma xenografts derived from U-87 MG-pLV-LASS2 cells compared with those derived from U-87 MG-pLV cells (Fig. 5G).