It was noted that LASS2 overexpression in CD133+ U-87 MG cells inhibited GSC sphere formation and reduced the protein levels of Notch1, CD133, Nestin, and Sox2, thus implying that LASS2 can influence glioma stem cell-specific proteins and inhibit stemness maintenance of GSCs, and thus reduce the growth of GSCs-derived glioblastoma growth. This evidence concerns the gene NOTCH1 and glioma.