To validate the function of ALKBH5 as a tumor suppressor in cells, we construct BE(2)-C-shALKBH5-Tet-on STCs and found that 1μg/mL of Dox was capable to induce ALKBH5 knockdown in both mRNA level (Figure 7F) and protein level (Figure 7G). This evidence concerns the gene ALKBH5 and neoplasm.