The studies showed that shXAF1 treatment not only reduced XAF1 expression (Fig. 2A and B) but also reduced glioma cell apoptosis (Fig. 2C and D) induced by WZY-321, indicating that WZY-321 lead to glioma cell apoptosis through up-regulating XAF1 expression. This evidence concerns the gene XAF1 and central nervous system cancer.