To explore the role of XAF1 gene in glioma cell apoptosis in response to WZY-321, the cultured glioma cells were divided into the following 3 groups, (1) shCTR, (2) shCTR + WZY-321, (3) shXAF1 + WZY-321. The gene discussed is XAF1; the disease is central nervous system cancer.