To further test whether WZY-321 can affect XAF1 gene transcription, the glioma cells were transfected with pGL3-XAF1-full-length (pGL3-XAF1-FL) for 48h, and then the glioma cells were treated with WZY-321 for another 9h, 12h or 24h. This evidence concerns the gene FLT3LG and central nervous system cancer.