In this study, we investigated the regulation of Notch1 expression on the stem cell-like characteristics and sensitivity to chemotherapeutic drugs VM-26 and BCNU in gastric cancer SGC-7901 cells by transgenic regulation of Notch1 signaling, and the results showed that when Notch1 signaling was enhanced, SGC-7901 cells showed stronger characteristics of tumor stem cells, such as CD133+ tumor cells. Here, NOTCH1 is linked to gastric cancer.