The results showed that when Notch1 signaling was enhanced, SGC-7901 cells showed stronger characteristics of tumor stem cells, such as CD133+ tumor cells The results showed that SGC-7901 cells showed stronger characteristics of tumor stem cells, such as more CD133+ tumor cells, increased nestin expression and decreased GFAP expression, increased tumor sphere formation, increased tumorigenicity of SCID mice subcutaneously transplanted, and significantly increased resistance to both VM-26 and BCNU when Notch1 signaling was enhanced. This evidence concerns the gene NOTCH1 and neoplasm.