We next investigated ASPM expression based on cancer stage and found that the expression levels of ASPM were significantly higher in late-stage cancers than in early stages of cancer for KIRC, KIRP, LIHC, LUAD and BRCA (Figure 2, p-values < 0.05).The expression levels of ASPM were significantly higher in stage 3 than stage 1(p < 10-3), stage 4 than stage 2 (p < 10-3), stage 4 than stage 3 (p < 10-2) in KIRC. This evidence concerns the gene ASPM and cancer.