The current study showed that maternal supplementation of LiCl during a relatively short period (from E7.5 to E9.5) can reduce the frequency and severity of spinal NTDs, with ∼18% of Lrp6-cKO mutants exhibiting completely closed neural tubes and an additional 20% with reduced NTD lesion sizes in the rescue group. The gene discussed is LRP6; the disease is neural tube defect.