We first screened four mutations, namely, ANO3/MUC15 rs15783, COL4A4 rs1800517, RRBP1 rs11960, and KLK1 rs5516, which were identified to be associated with COPD via a WES analysis of a cluster of three COPD families. The gene discussed is COL4A4; the disease is chronic obstructive pulmonary disease.