To further investigate whether the cytotoxicity induced by RWF extract is due to the increase in apoptosis, we treated both BPH-1 and WPMY-1 cells with 0, 125, 250, and 500 μg/ml of RWF extract for 72 h, followed by Annexin V/PI double staining assay. Here, ANXA5 is linked to benign prostatic hyperplasia.