To further understand the difference between cancer-promoting mechanisms of LncRNA MAFG-AS1 in nucleus and cytoplasm, and in order to study the functional mechanism of lncRNA MAFG-AS1, nuclear and cytoplasmic separation assay was exploited to detect its location in cells, which revealed that LncRNA MAFG-AS1 located in cytoplasm primarily in MDA-MB-231 and T-47D cells (Fig. 9A, B), and was colocalized with STC2 (Fig. 9C, D). Here, MAFG is linked to cancer.