As the results of colony formation assays demonstrated that knockdown of LncRNA MAFG-AS1 in MDA-MB-231 and T-47D could suppress the breast cancer colony viability visibly (Fig. 2E), while over-expressing LncRNA MAFG-AS1 could promote the breast cancer cells proliferation activity overtly (Fig. 2F). This evidence concerns the gene MAFG and breast cancer.