Low allele frequencies (< 0.3) suggested that Dis3 and Rara mutations were subclonal, whereas a single copy of Ctnnb1 1004A>C (K335T substitution) with an allele frequency of 0.48 was likely to be clonal and thus possibly involved in tumor initiation (Fig 5A, Appendix Fig S5A). The gene discussed is CTNNB1; the disease is neoplasm.