Lastly, we also established pancreatic tumor-bearing xenograft nude mice to validate the oncogenic role of miR-331-3p in vivo, and our data revealed that miR-331-3p considerably increases the tumor size, volume, and weight in nude mice and promotes the tumorigenesis of PC through SCARA5/FAK axis in vivo. This evidence concerns the gene SCARA5 and pancreatic neoplasm.