Additionally, to confirm that LA affected proliferation, colony formation, and 3D tumor microsphere formation of MKN-45 cells by blocking the binding of VEGF and VEGFR-2, MKN-45 cells were stimulated with 20 ng/mL VEGF and then treated with 0, 25, 50, and 100 μM LA for 72 h. The gene discussed is VEGFA; the disease is neoplasm.