In addition, restricted cubic spline with a multivariate logistic regression model demonstrated that Klotho level had a linear association and a negative correlation with CHF (P for non-linearity = 0.754; Figure 1), whereas no relationship between Klotho level and coronary heart disease, angina, heart attack, or stroke was significant (Supplementary Figures 3A-D). This evidence concerns the gene KL and Stroke.