In addition, we also discovered that the ubiquitin-associated protein P62, interacts with TPI1 and promotes ubiquitin-dependent proteasome degradation of TPI1 in MDA-MB-231cells.Our study not only demonstrates, for the first time, the role of TPI1 in promoting breast cancer progression, but also provides a new strategy for targeted glycolysis therapy for breast cancer. Here, TPI1 is linked to breast carcinoma.