To further clarify whether the low expression of FOXC1 mediated by the FOXC1 H446HG mutation causes abnormal differentiation of Tregs through the KLFs family dependent signaling pathway, we further detected the expression of KLFs (KLF1, 2, 4, 5, 10 and 13) in the T cells of FOXC1 mutant patients with ALL and healthy controls. This evidence concerns the gene KLF1 and acute lymphoblastic leukemia.