By evaluating a combination of tumor, leading edge, and normal samples, Zheng et al. found that the CD4 and CD8 double-positive T (DPT) cell cluster was enriched in the leading-edge region;160 however, this cluster generally exists in the thymus.161 The DPT cluster was divided into 11 subclusters based on canonical markers including cytotoxic DPT, memory DPT, activated DPT, NK-like DPT, MAIT-like DPT, and exhaustion DPT. The gene discussed is CD8A; the disease is neoplasm.