CD4 and neoplasm: Lee et al. also found that Th17 cells and Tregs were predominantly present at the tumor site, whereas γδT cells were enriched at the normal tissue site.182 Zhang et al. observed that CD8+ T cells, Th1/Th2 cells, and memory T cells were increased at the tumor site, whereas CD4+ T cells and Tregs were decreased.183 Based on enrichment analysis results, the imbalance in T cell clusters may be affected by T cell proliferation, activation/differentiation, and TCR signaling.