MRGPRF and neoplasm: To validate the downregulation of MrgprF in CM, both the mRNA and protein expressions of MrgprF in cancerous cell lines (A875, A375, SK-MEL-1, and SK-MEL-5) and immortalized human keratinocyte-HaCaT cells was examined, and MrgprF was revealed to be markedly decreased in tumor cells (Fig. 1k).