Finally, we performed RNA-Seq on distal colon tumors from littermate- and cage-matched WT and Mtg16T/T mice to test whether Mtg16T/T AOM/DSS tumors would exhibit the increased canonical WNT signaling tone that we previously demonstrated in Mtg16–/– AOM/DSS tumors (18). This evidence concerns the gene CBFA2T3 and digestive system neoplasm.