Based on this theory, in this study, we tried to generate a new animal model of schizophrenia for which we treated methotrexate (MTX), a DHFR inhibitor, to pregnant rats to inhibit folate metabolism and evaluated offspring behaviors associated with schizophrenia (prepulse inhibition, social interaction, and locomotor activity; as primary outcomes in this study). Here, DHFR is linked to schizophrenia.