To observe the effect of gene expression on tumors, we divided the rectal cancer samples into two groups with high and low expression according to the median expression levels of CYCS, NFKB1, IKBKB, and TRADD and analyzed the enrichment of signaling pathways in KEGG and HALLMARK in high- and low-expression groups by GSEA. Here, NFKB1 is linked to rectal cancer.