The top 7 pathways were significantly different in the results of KEGG, which were Alcoholism, Systemic lupus erythematosus (SLE), Viral carcinogenesis, NF-kappa B signaling pathway, Transcriptional misregulation in cancer, TNF signaling pathway and NOD-like receptor signaling pathway, as shown in Additional file 1: Table S2. The gene discussed is TNF; the disease is alcohol dependence.