The TgF344-AD hippocampal molecular layers similarly exhibited no overall genotype effect (P = 0.84), with a significant loss over age (P < 0.0001) and an age × genotype interaction (P = 0.0002); notably, a recovery of GAD67 immunoreactivity in 12-month-old TgF344-AD rats was observed, compared to NTg (P = 0.001; Fig. 2e; Table 2). This evidence concerns the gene GAD1 and Alzheimer disease.