Both NTg and TgF344-AD rats consistently exhibited age-related loss of NeuN+ in layer II entorhinal cortical neurons (P < 0.0001), with significantly less neurons in TgF344-AD rats compared to NTg (overall genotype comparison across all ages: P = 0.03), suggesting that this age-related loss began earlier in TgF344-AD than in NTg rats. This evidence concerns the gene RBFOX3 and Alzheimer disease.