EVs derived from pancreatic cancer cell lines incubated with non-polarized macrophages (THP-1 cells) caused higher expression of surface proteins CD14, CD163, and CD206, as well as higher secretion of pro-tumorigenic factors like VEGF, MCP-1, IL-6, IL-1β, MMP-9, and TNF-α. The gene discussed is TNF; the disease is familial pancreatic carcinoma.