The downregulation effect of ZBTB7A on HIC1 expression in BC cells was assessed by cloning the promoter regions of HIC1 into pGL3-based luciferase reporter plasmids, which were labeled P1-P3 (Fig. 2K), and then transfecting these plasmids into 293 T cells with or without ZBTB7A overexpression. This evidence concerns the gene HIC1 and breast cancer.