ASCL1 and neoplasm: Whereas the vast majority of SHH-1B [10/12 (83%)] stained positive for ASCL1 (> 5% of tumor cells), positive ASCL1 staining was less frequent in SHH-1A [7/23 (30%) and SHH-2 [9/21 (43%); Chi-Square: 9.044; P = 0.01].