Endogenous immunoprecipitation (IP) in HCC cells revealed that the major components of the NuRD complex, chromodomain helicase DNA binding protein 4 (CHD4) and HDAC1, were detected in the immunoprecipitates, confirming their interaction (Figs. 6a and S4a, b). This evidence concerns the gene CHD4 and hepatocellular carcinoma.