To explore the biological function of UHMK1 in CRC, a transient transfection model using siRNAs and a stable transfection model using lentivirus-mediated shRNA in RKO cells and HCT116 cells were established, which successfully downregulated the expression of UHMK1 (Fig. 2B). This evidence concerns the gene UHMK1 and colorectal carcinoma.