Importantly, the frequencies of multifunctional CFA-specific T cells producing IFN-γ+TNF-α+IL-2+IL-17A+, IFN-γ+TNF-α+IL-2+, IFN-γ+TNF-α+IL-17A+, IFN-γ+IL-2+IL-17A+, IFN-γ+TNF-α+, IFN-γ+IL-2+, and IFN-γ+IL-17A+ in the lungs and those of IFN-γ+TNF-α+IL-2+ producing T cells in the spleens of the CFA+GLA-SE/CDG-immunized group were highly detected compared to those in the CFA+GLA-SE-immunized and control groups (Figure 4c,d and Supplementary Figure S6C,D), suggesting that the CFA+GLA-SE/CDG immunization-derived multifunctionality of CD4+ T cells was durably sustained at 10 weeks post-infection. This evidence concerns the gene IFNG and infection.