The frequencies of the multifunctionality of CD4+ T cells, including those producing Th1- and Th17-associated cytokines such as IFN-γ, IL-2, and IL-17A but not TNF-α, were increased, but those of cells expressing IL-10 were decreased in the lung and spleens in the CLR+CFA+GLA-SE/CDG group with ex vivo Mav CFA stimulation, compared to those in the infection control group. This evidence concerns the gene CD4 and infection.