To extend our characterization of the tumor immune microenvironment, we utilized an RT-qPCR array to analyze mRNAs encoding 84 cytokines, chemokines, interleukins, and other secreted factors, which revealed decreased expression of 40 mRNAs, including those encoding the immunosuppressive cytokines CXCL1, IL-4, IL-6, IL-10, IL-13, and VEGFA (Supplemental Table 4), and increased expression of 5 mRNAs, including CXCL9 and CXCL10, in tumors from 32-134D–treated mice (P < 0.05; Figure 6E and Supplemental Table 3). This evidence concerns the gene CXCL1 and neoplasm.