CXCL1 and neoplasm: To further extend these findings, protein expression was quantified by performing ELISAs on tumor lysates, which confirmed that tumors from 32-134D–treated mice contained significantly increased levels of CXCL2, CXCL9, and CXCL10 (P < 0.05; Figure 7, A–C), which are chemokines that promote antitumor immunity, as well as decreased levels of CXCL1, IL-6, IL-10, and VEGFA (Figure 7, D–H), which are secreted factors that promote establishment of an immunosuppressive tumor microenvironment (Supplemental Table 4).