Moreover, MVD and tumor cell proliferation assessment in mouse models of BC exhibited that miR-145 overexpression resulted in decreased MVD and tumor growth by targeting VEGF, insulin receptor substrate (IRS), and N-RAS directly, as well as, important mediators in the PI3K/AKT signaling pathway such as AKT, mTOR, phosphatidylinositol-4,5-bisphosphate 3-Kinase catalytic subunit alpha (PIK3CA), and phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) indirectly [86]. The gene discussed is PIK3CA; the disease is breast cancer.