Further, supporting the possible involvement of Tlr4 in mediating cytokine induction, we report here that DHA enrichment of the diet, which prevented hypothalamic increase of cytokines also prevented the increase of Tlr4. We also found a decrease in the gene expression of malonyl-CoA decarboxylase in pituitaries but not hypothalamus of rats fed HF diet. The gene discussed is TLR4; the disease is hydrops fetalis.