ING4 and renal cell carcinoma: Meanwhile, we further produced three lentivirus-mediated sgRNAs to stably knock down ING4 expression in these two RCC cells (Figures 1E,F), and under ultralow-attachment culture conditions, we found that ING4 knockdown significantly reduced the sphere-forming size and number in RCC cells compared to the respective controls (Supplementary Figure S1B and Figures 1G,H).