We pretreated Ketr-3 and 786-O cells with 5 μM SB203580 or 1 μM BIRB0796 for 24 h, and then examined the cell sphere formation, and found that SB203580 and BIRB0796 showed no significant inhibitory effect on vector cells, but almost completely impaired ING4 overexpression-promoted number and size of cell spheres formed by RCC cells (Figures 5E,F, Supplementary Figures S3, S4). The gene discussed is ING4; the disease is renal cell carcinoma.