Most of the submutations classified as subclone2 showed poorer OS, such as AHNAK and AHNAK2 in GBM (Supplementary Figure 4A-C for AHNAK2) and CIC and FLG in LGG (Supplementary Figure 4D-F for CIC), further supporting the prognostic value of subclonal mutations. The gene discussed is AHNAK2; the disease is glioblastoma.