When comparing with the risk subclonal mutations, we found that the presence of a subclonal mutation was an independent factor (p value = 0.0375 in GBM and 7.88e-06 in LGG, multivariate Cox regression analysis) and could separate patients with IDH mutation into two subgroups with significant differences in OS (p value < 0.001 in both GBM and LGG, log-rank test, Figures 3(e) and 3(f)). The gene discussed is IDH1; the disease is glioblastoma.