Since the levels of phosphorylated MET are very low in untreated cells, in order to better analyze the interaction between SMYD3 and phospho-activated MET, we treated HT-29 CRC cells with 10 ng/ml of HGF for 2 h before performing the co-immunoprecipitation experiments [99], [100]. Here, SMYD3 is linked to colorectal carcinoma.