NEFL and Wolfram syndrome: One individual with Wolfram syndrome had raw plasma NfL levels at both time points 1 (37.8 pg/mL) and 2 (34.2 pg/mL) >3 SD above the respective means, but were < 3SD above log10 means at time points 1 (log10 plasma NfL = 1.58) and 2 (1.53) within the Wolfram group and when controls were included in the calculation.