To assess the potential consequence of combined inhibition of CK1δ/ε and Tip60 activities, we tested the effect of combined treatment with CK1δ/ε inhibitor SR3029 and Tip60 inhibitor MG149 on β-catenin acetylation, the transcription of Wnt target genes, cell viability and proliferation in colon cancer cells. This evidence concerns the gene KAT5 and colonic neoplasm.