Taken together, these results illustrated that silencing of CK1δ/1ε or treatment with CK1 inhibitor could reduce the β-catenin acetylation and suppress β‐catenin binding to the promoters of Wnt target genes, resulting in the inhibition of colon cancer cell viability and proliferation. The gene discussed is CSNK1A1; the disease is malignant colon neoplasm.